Arbuscular mycorrhizal (AM) fungi form obligate symbioses with most plants species. The plants benefit from the association via improved mineral uptake and water relations while the fungus receives fixed carbon from the plant. We are utilizing metabolite profiling and functional genomics to broaden our knowledge of metabolic pathways operating in Glomus intraradices. We are testing models of metabolism in germinating spores and extraradical mycelia (ERM).  This project is a collaboration between the laboratories of Dr. Yair Shachar-Hill at Michigan State University, and Dr. Peter Lammers at New Mexico State University.

RESOURCES ON THIS PAGE

Gene Discovery:  Random cDNA sequencing from a germinating spore library have indentified many metabolic genes.  Full access to data on ESTs and metabolic clones can be viewed using the links provided below.  

• View table of genes indentified via random cDNA sequencing and BLASTX analysis: Gene Table

• 563 ESTs from extraradical mycelia from Glomus intraradices.  Sortable view of BLAST annotation results made using the Southwest Biotechnology and Informatics Center (SWBIC) Batch BLAST service.  Sequences provided by Dr. Maria Harrison. View data sorted by number of BLAST hits against the November 2001 GenBank NR database or by descending "E-Value".

• 1083 ESTs from 10 day germinating spores from Glomus intraradices. Sortable view of BLAST annotation results. Sequences from random sequencing of 10 day germinating spore clones.

• Glyoxylate Cycle Genes isolated using degenerate PCR primers: Isocitrate lyase, Malate synthase.  P.J. Lammers, J. Jun, J. Abubaker, R. Arreola, A. Gopalan, B. Bago, C. Hernandez-Sebastia, J.W. Allen, D.D. Douds, P.E. Pfeffer, and Y. Shachar-Hill (2001) The Glyoxylate Cycle in an Arbuscular Mycorrhizal Fungus. Carbon Flux and Gene Expression. Plant Physiol. 2001 127: 1287-1298

Batch BLAST results for blastx of genomic library against non-redundant protein database, 27 May 2003.

 Movies of lipid movement in Arbuscular Mycorrhizal Fungi by Berta Bago.  Carbon taken up from the plant as hexoses is converted to triacyclglycerols found in lipid bodies which move from the intraradical mycelia to the extraradical mycelia.

Gallery of images: Find images of  AM fungi, ectomycorrhiza, and other mycorrhizae at Mycorrhizal Image Exchange  

 

 

 

 

 

 

 

 

Glomus Project Personnel